PUBLICATION DETAILS

Phylogenetic and genome wide deep sequencing analyses of canine parvovirus reveal co-infection with field variants and emergence of a recombinant strain.

Perez, R; Calleros, L; Marandino, A; Sarute, N; Iraola, G; Grecco, S; Blanc, H; Vignuzzi, M; Isakov, O; Shomron, N; Carrau, L; Hernández, M; Francia, L; Sosa, K; Tomás, G & Panzera, Y

PLoS ONE 02/12/2020

PMID: 25365348

Abstract

Abstract: Canine parvovirus (CPV), a fast-evolving single-stranded DNA virus, comprises three antigenic variants (2a, 2b, and 2c) with different frequencies and genetic variability among countries. The contribution of co-infection and recombination to the genetic variability of CPV is far from being fully elucidated. Here we took advantage of a natural CPV population, recently formed by the convergence of divergent CPV-2c and CPV-2a strains, to study co-infection and recombination. Complete sequences of the viral coding region of CPV-2a and CPV-2c strains from 40 samples were generated and analyzed using phylogenetic tools. Two samples showed co-infection and were further analyzed by deep sequencing. The sequence profile of one of the samples revealed the presence of CPV-2c and CPV-2a strains that differed at 29 nucleotides. The other sample included a minor CPV-2a strain (13.3% of the viral population) and a major recombinant strain (86.7%). The recombinant strain arose from inter-genotypic recombination between CPV-2c and CPV-2a strains within the VP1/VP2 gene boundary. Our findings highlight the importance of deep-sequencing analysis to provide a better understanding of CPV molecular diversity